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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
at-20℃
- 适应物种:
Crotalus durissus terrificus (South American rattlesnake), Crotalus scutulatus scutulatus (Mojave rattlesnake)
- 库存:
99
- 宿主:
Mouse
- 应用范围:
ELISA,FCM,IF,SPR,WB
- 浓度:
见说明
- 抗体英文名:
Anti-South American/Mojave rattlesnake crotoxin Monoclonal antibody (Al0G)
- 规格:
50-100ug
小鼠抗South American/Mojave rattlesnake crotoxin单克隆抗体(克隆号Al0G)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

小鼠抗South American/Mojave rattlesnake crotoxin单克隆抗体(克隆号Al0G)更多优势产品欢迎新老客户选购。
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MAB1600 小鼠抗ASFV I10L单克隆抗体(克隆号1A016)
MAB1601 小鼠抗ASFV NS4A单克隆抗体(克隆号1A017)
MAB1602 小鼠抗ASFV NS4A单克隆抗体(克隆号1A018)
MAB1603 小鼠抗ASFV F778R单克隆抗体(克隆号1A019)
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文献和实验对照细胞有以下几种: 1)非染色细胞:直接染色法时,用10μl PBS代替与荧光结合的单克隆抗体,其它步骤相同。间接染色法时,分别用10μl的PBS代替第一抗体和荧光第二抗体,其它步骤相同。 2)使用非特异性的抗小鼠膜表面免疫球蛋白(MsIg),代替特异性的单克隆抗体。这是由于所使用的单克隆抗体来自小鼠,可能造成非特异性的假性反应,因此使用MsIg作为对照。同时还需要注意选择与实验用单克隆抗体亚类一致的MsIg。如大部分单克隆抗体为IgG1,也有部分抗体为IgM,所以用MsIg作对照组时
瘤细胞。将单个杂交瘤细胞分离,在体内或体外培养而分泌的抗体单克隆抗体(monoclonal antibody,McAb或Mab)。单克隆抗体仅针对一种抗原决定簇,具有很高的特异性。单克隆抗体通常用抗原免疫小鼠制备。将免疫的脾细胞(含产生抗体的B细胞)与小鼠肿瘤细胞融合,分离杂交瘤细胞,接种于小鼠腹腔,产生的腹水中含有浓度很高的单克隆抗体。1.3 抗原抗体反应1.3.1 可逆性 抗原与抗体结合形成抗原抗体复合物的过程是一种动态平衡,其反应式为:Ag+Ab→Ag·Ab。.抗体的亲和力(affinity)是抗原
和PBS含正常羊血清(1:30),室温1h,或4℃过夜。 (4)PBS彻底冲洗。 (5)载片加生物素化抗小鼠IgG(1:100)30min室温。 (6)PBS彻底冲洗。 (7)应用ABC复合物与等量的1%牛血清白蛋白-PBS液混合孵育1h,室温。 (8)PBS彻底冲洗。 (9)将切片覆以新鲜配制的0.025%DAB溶液,0.02%H2 O2 ,孵育3~15min。 (10)水洗,复染,脱水,透明和以甘油/PBS或DPX封固。如需要可用银染,多层
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