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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgM
- 形态:
Liquid
- 保存条件:
at-20℃
- 适应物种:
Human enterovirus 71 (EV71) (EV-71)
- 库存:
99
- 宿主:
Human
- 应用范围:
ELISA,IP,Neutralization
- 浓度:
见说明
- 抗体英文名:
Anti-EV71 Capsid protein VP2/VP2 Monoclonal antibody (M1-20)
- 规格:
50-100ug
抗EV71 Capsid protein VP2/VP2单克隆抗体(克隆号M1-20)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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文献和实验第一作者之一的宋尔卫(Erwei Song)是我们熟知的,他是中山大学附二院的医生,现在哈佛大学医学院血液研究中心Judy Lieberman教授组进行博士后研究。他曾发表用尾静脉快速大剂量法向小鼠体内输注siRNA成功实现耐受抗Fas单抗诱导的暴发性肝坏死。 http://www.nature.com/nbt/journal/v23/n6/abs/nbt1101.html;jsessionid=EFF86FD58B3B57397768DE431AACF68D Nature
capsid in insect cells infected with recombinant baculovirus expressing HSV capsid protein. J Virol, 1994; 68:2442-2457. 4 Newcomb WW, Brown JC. Induced extrusion of DNA from the capsid of HSV-1. J Virol, 1994; 68:434-440. 5 洪涛.病毒的形态学《医学病毒
和核碎片。上清液与SDS-PAGE样品缓冲液混合,100°C加热5分钟,14mA 10%SDS-PAGE凝胶分离过夜。用一个Trans-Blet SD 半干转移槽(Bi-Rad)转移蛋白质到硝酸纤维素膜上。滤膜在PBS溶解的2%奶粉阻断1-2h,随后用兔抗Rep(Trempe等,1987)或仓鼠抗Cap(American Type Culture Collection)一抗(含有1%奶粉的PBS稀释200倍)室温孵育2小时或4°C过夜。印迹用含有0.05% Tween-20的PBS洗涤3次,每次
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