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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
at-20℃
- 库存:
99
- 宿主:
Mouse
- 应用范围:
IHC,WB
- 浓度:
见说明
- 抗体英文名:
Anti-GABA(A)R, Alpha5 Monoclonal antibody (N415/31)
- 规格:
50-100ug
小鼠抗GABA(A)R, Alpha5单克隆抗体(克隆号N415/31)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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TNF-α抗体,用于治疗风湿性关节炎和节段性回肠炎。4、SimulectNovartis公司的抗CD25抗体,用于抗移植排斥。5、Erbitux美国Imclone公司的抗EGFR(Her-1)单克隆抗体,目前FDA已批准其用于治疗结直肠癌。6、人源化抗体利用现有的无数已详细分析过的小鼠抗体,取其与抗原直接接触的那段抗体片段(互补决定区,CDR)与人的抗体框架嫁接,经亲和力重塑,可维持其特异性和大部分的亲和力,同时几乎去除免疫原性和毒副作用。7、成功的例子(1)HerceptinGenentech公司的抗
后能形成肿瘤,即所谓杂交瘤。生长杂交瘤的小鼠血清和腹水中含有大量同质的抗体,即单克隆抗体。 这一技术建立后不久,在融合剂和所用的骨髓瘤细胞系等方面即得到改进。最早仙台病毒被用做融合剂,后来发现聚乙二醇(PEG)的融合效果更好,且避免了病毒的污染问题,从而得到广泛的应用。随后建立的骨髓瘤细胞系如SP2/0-Ag14,X63-Ag8.653和NSO/1都是既不合成轻链又不合成重链的变种,所以由它们产生的杂交瘤细胞系,只分泌一种针对预定的抗原的抗体分子,克服了骨髓瘤细胞MOPC-21等的不足。再后来
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