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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
at-20℃
- 库存:
99
- 宿主:
Mouse
- 应用范围:
IHC,WB
- 浓度:
见说明
- 抗体英文名:
Anti-nNOS/NOS1 Monoclonal antibody (L121/42)
- 规格:
50-100ug
小鼠抗nNOS/NOS1单克隆抗体(克隆号L121/42)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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大于 109 计数/分/μl。六、杂交Southern 杂交一般采取的是液-固杂交方式,即探针为液相,被杂交 DNA 为固相。杂交发生于一定条件的溶液(杂交液)中并需要一定的温度,可以用杂交瓶或杂交袋并使液体不断的在膜上流动。杂交液可以自制或从公司购买,不同的杂交液配方相差较大,杂交温度也不同。下面给出的为一杂交液配方:PEG 6000 10%;SDS 0.5%;6×SSC;50% 甲酰胺。 该杂交液的杂交温度为 42 ℃。1.预杂交NC 膜浸入 2× SSC 中 5 min,在杂交瓶中加入杂交
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