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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
at-20℃
- 库存:
99
- 宿主:
Mouse
- 应用范围:
IHC,WB
- 浓度:
见说明
- 抗体英文名:
Anti-BDNF Monoclonal antibody (N450/53)
- 规格:
50-100ug
小鼠抗BDNF单克隆抗体(克隆号N450/53)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠脑衍化神经营养因子 ( BDNF )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 BDNF 单抗包被于酶标板上,标准品和样品中的 BDNF与单抗结合,加入生物素化的抗小鼠 BDNF ,形成免疫复合物连接在板上,辣根
小鼠脑衍化神经营养因子受体(BDNF R)ELISA试剂盒 说明书
,加入生物素化的抗小鼠 BDNF R ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,BDNF R 浓度与 OD 值成正比,可通过绘制标准曲线求出标本中BDNF R 浓度。 试剂盒组成 ( 2-8 ℃保存) 酶标 板 (Coated Wells ) 96
小鼠总抗氧化能力的测定 刘小美 宋菊敏 (2006-10-24) 一、原理 机体中有许多抗氧化物质,能使Fe3 + 还原成Fe2 + ,后者可与菲啉类物质形成稳固的络合物,通过比色可测出其抗氧化能力的高低。 二、目的 1.掌握总抗
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