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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
at-20℃
- 适应物种:
Human enterovirus 71 (strain 7423/MS/87) (EV71) (EV-71)
- 库存:
99
- 宿主:
Human
- 应用范围:
ELISA,FCM,IP,Neutralization
- 浓度:
见说明
- 抗体英文名:
Anti-EV71 Capsid protein VP1/2/3 Monoclonal antibody (SAA2027)
- 规格:
50-100ug
抗EV71 Capsid protein VP1/2/3单克隆抗体(克隆号SAA2027)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

抗EV71 Capsid protein VP1/2/3单克隆抗体(克隆号SAA2027)更多优势产品欢迎新老客户选购。
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文献和实验[6]。以CD20作为治疗B淋巴细胞瘤的理想靶点具有很好的选择性。HI47(IgG3)是我所于1990年研制成功的国内第一个抗CD20鼠源性单克隆抗体,第四届国际人类白细胞分化抗原会议将HI47命名为CD20+X[7]。我们利用噬菌体显示技术克隆到HI47可变区基因,利用该基因构建了嵌合抗CD20 Fab片段,并在大肠杆菌中可溶性分泌表达[8],本研究旨在研究抗CD20 Fab抗肿瘤活性的机制。一、材料与方法1、材料PI为Sigma公司产品;3H-UdR购于中国原子能研究所;Raji细胞为我室所存,用含小牛
因为抗体的结合而减少;在人体血清中无游离的CD20存在;最重要的是B淋巴细胞瘤上的CD20少有内吞和脱落的发生[ 2-5 ] 。1997年,以CD20为靶点的嵌合抗CD20单克隆抗体Rituxan已获准应用于B细胞淋巴瘤的治疗[ 6 ]。HI47(IgG3)是我所于1990年研制成功的国内第一个抗CD20鼠源性单克窿抗体,第四届国际人类白细胞分化抗原会议将HI47命名为CD20+X[ 7 ] 。我们利用噬菌体显示技术从HI47杂交瘤细胞中克隆到抗CD20抗体HI47可变区基因,将该基因构建成为嵌合的抗
,用含有10%热灭活胎牛血清的RPMI1640培养基于5% CO2,37C条件下培养。抗CD20单克隆抗体HI47由我所免疫室提供。辣根过氧化酶标记羊抗人IgG,FITC标记的羊抗鼠IgG购于北京中山生物工程公司。2、抗CD20单克隆抗体轻、重链基因的克隆利用RT-PCR法,采用抗体通用的兼并性引物,从HI47杂交瘤细胞特异性地分别扩增抗体轻链、重链可变区基因(VL和VH)后,利用编码短肽(G4S)3的连接链DNA片段,经overlap PCR,将VH和VL基因连接构成ScFv基因(约750bp
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