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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
at-20℃
- 适应物种:
Human
- 库存:
99
- 宿主:
Mouse
- 应用范围:
ELISA
- 浓度:
见说明
- 抗体英文名:
Anti-Human ACTN4/Actinin alpha 4 Monoclonal antibody (SAA1752)
- 规格:
50-100ug
小鼠抗人ACTN4/Actinin alpha 4单克隆抗体(克隆号SAA1752)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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文献和实验隆 抗体 在治疗类风湿关节炎、系统性红斑狼疮、局限性回肠炎、多发性硬化症等 有独到之处。经FDA 批准用于治疗类风湿关节炎的名为infliximab 药物即是一种由人Fc 段和鼠Fab 段 嵌合而成的抗TNF -α单克隆抗体,其可明显改善类风湿关节炎临床症状并缓解关节破坏。
,室温搅拌作用1小时,以封闭HRP分子上的α和ε氨基。 (2)再加1ml 0.06mol/L 过碘酸钠溶液,在室温中避光轻搅30分钟,溶液呈黄绿色;随后加1ml 0.06mol/L 乙二醇,轻搅1小时,中止氧化反应。 (3)移入透析袋中,在1000ml 0.01mol/L PH9.5碳酸盐缓冲液中,4℃透析过夜,更换三次缓冲液,注意避光。 (4)吸取上述醛化好的HRP溶液3ml,加入5mg IgG的碳酸盐缓冲液1ml,室温轻搅2-3小时
相关专题 层析柱的类型及作用 重组 人α干扰素作为一种具有广谱抗病毒、抑制肿瘤细胞生长及免疫调节作用的细胞因子新型药物,已在国内外广泛投入临床应用,并取得了显著的疗效。国际上对用于临床的重组干扰素的纯度要求很高,美国NIH规定必须在90%以上,因此生产者普遍采用亲和层析纯化法。我们研制了rHuIFN-α2a的McAb 3F1,与CNBr活化的Sepharose 4B交联制成亲和层析柱,经1年60余次较大规模试用
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