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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
at-20℃
- 库存:
99
- 抗体英文名:
Anti-5HT3/HTR3A Monoclonal antibody (SAA1479)
- 规格:
50-100ug
小鼠抗小鼠5HT3/HTR3A单克隆抗体(克隆号SAA1479)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

小鼠抗小鼠5HT3/HTR3A单克隆抗体(克隆号SAA1479)更多优势产品欢迎新老客户选购。
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文献和实验抗大鼠波形蛋白单克隆抗体,l:200稀释度),4℃过夜或37℃孵育30分钟,BS漂洗3次,每次5分钟; (7)滴加与第一抗体种属匹配的荧光素标记二抗(如羊抗小鼠FITC-IgG。1:100),37℃避光孵育30分钟,PBS漂洗3次,每次5分钟; (8)Hoechst 333442复染细胞核15~20分钟(不是必需步骤); (9)甘油磷酸缓冲液封片,荧光显微镜下镜观察; (10)结果分析:波形蛋白免疫反应阳性细胞胞质呈现绿色荧光,如细胞核复染后可呈现亮蓝色荧光。阴性细胞仅见细胞
有好几个同仁发消息问CD31染色,刚工作比较忙,今天分享一下我做的是免疫荧光,也试做过免疫组化,都可以做出来抗体:小鼠抗CD31单克隆抗体 英国Abcam公司(CD31抗体说明书里面有说多聚甲醛会掩盖抗体,所以推荐速冻切片,也尝试过速冻切片,不使用多聚甲醛,但是这个技术个人掌握不好,所以还是用了多聚甲醛固定)二抗: 山羊抗小鼠488单克隆抗体 美国CST公司 特别说明:我是从同学那里得到的经验,可能绿光稍微好做些,之前做红光
R&D Systems:如何提高MSC的扩增且不损失其分化潜能
- M E M 培养基(Invitrogen,Carlsbad)。将1.25×105量级的hMSCs接种于一个T75长颈瓶,在细胞汇聚之前让细胞生长3至4天。在培养期末,培养于不同条件的hMSCs同时用胰蛋白酶/EDTA溶液收集。对细胞计数,并且用下面的公式计算扩增倍数。重新培养时,将1.25×105量级的hMSCs接种于一个新的T75长颈瓶,并用适当的培养基培养。每次传代后对细胞进行计数。 抗体。藻红蛋白(PE)连接的小鼠抗人CD90和CD105的单克隆抗体(mAbs),PE非连接的山羊抗小鼠
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