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- 文献和实验
- 技术资料
- 保存条件:
at-20℃
- 库存:
99
- 抗体英文名:
Anti-Human GIPR/GIP-R Monoclonal antibody (SAA1449)
- 规格:
50-100ug
小鼠抗人GIPR/GIP-R单克隆抗体(克隆号SAA1449)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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文献和实验R&D Systems:如何提高MSC的扩增且不损失其分化潜能
。人MSCs (hMSCs ) 购于Cambr e x ( Lonz a ,Walkersville),先根据供应商的说明书培养,然后用StemXVivo人/小鼠骨髓间充质干细胞扩增培养基( 目录# C C M 0 0 4 ; R & DSystems,Minneapolis)培养。为了测试不同批次FBS的扩增潜能,每种F B S 以终浓度为1 0 % 或2 0 % 加入含有谷氨酰胺, 丙酮酸钠( I r v i n e S c i e n t i c , S a n t a A n a ) 的α
分子。 表1-2 CD分子的主要特征 CD 常用单克隆抗体或代号( ) 主要表达细胞 分子量(kDa)和结构 功能 CDla T6,Leu6 Thy,DC,LHC gp49 TCRγδ的配体? CDlb WM-25,4A76,NUT2 Thy,LHC gp45 TCRγδ的配体? CDlc L161,M241,7C6 Thy,DC,Bsub gp43 TCRγδ的配体? CD2 9.6,T11,Leu5; (LFA-2,SRBC-R) T,NKsub. gp
分子。 表1-2 CD分子的主要特征 CD 常用单克隆抗体或代号( ) 主要表达细胞 分子量(kDa)和结构 功能 CDla T6,Leu6 Thy,DC,LHC gp49 TCRγδ的配体? CDlb WM-25,4A76,NUT2 Thy,LHC gp45 TCRγδ的配体? CDlc L161,M241,7C6 Thy,DC,Bsub gp43 TCRγδ的配体? CD2 9.6,T11,Leu5; (LFA-2,SRBC-R) T,NKsub. gp
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