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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
at-20℃
- 库存:
99
- 抗体英文名:
Anti-Bovine CD230/PRNP Monoclonal antibody (SAA2061)
- 规格:
50-100ug
小鼠抗Bovine CD230/PRNP单克隆抗体(克隆号SAA2061)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

小鼠抗Bovine CD230/PRNP单克隆抗体(克隆号SAA2061)更多优势产品欢迎新老客户选购。
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文献和实验有好几个同仁发消息问CD31染色,刚工作比较忙,今天分享一下我做的是免疫荧光,也试做过免疫组化,都可以做出来抗体:小鼠抗CD31单克隆抗体 英国Abcam公司(CD31抗体说明书里面有说多聚甲醛会掩盖抗体,所以推荐速冻切片,也尝试过速冻切片,不使用多聚甲醛,但是这个技术个人掌握不好,所以还是用了多聚甲醛固定)二抗: 山羊抗小鼠488单克隆抗体 美国CST公司 特别说明:我是从同学那里得到的经验,可能绿光稍微好做些,之前做红光
1)原位缺口翻译检测裂解的DNA片段 1.取106经促调亡物质处理的细胞,用1% BSA-PBS洗涤细胞后加入0.1ml FITC标记的抗CD4或抗CD8单克隆抗体,4℃ 20分钟。用1% BSA-PBS洗涤细胞。置冰上。 2.加入0.1ml 1%多聚甲醛,置冰上5分钟,加入60%(v/v)甲醇溶液,轻轻悬浮细胞,4℃固定细胞15分钟。4℃离心500×g 10分钟,去上清液。 3.加入
,4℃ 30分钟,用冷PBS洗涤细胞。 5.上样于FACS仪,用FACScan LysisⅡ软件分析。可以同时了解调亡细胞的类型。 二、末端脱氧核苷酸转移酶检测法检测裂解的DNA片段 1.在37℃,含10%小牛血清的RPMI-1640培养液中,用促调亡物质喜树碱处理人PBMC 4小时。或用10-5g/ml地塞米松处理小鼠胸腺细胞4小时。 2.取106经处理的细胞,用1% BSA-PBS洗涤细胞后加入0.1ml FITC标记的抗CD或抗CD8单克隆抗体
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