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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
at-20℃
- 库存:
99
- 抗体英文名:
Anti-Bet v 1/Allergen Bet v I-A Monoclonal antibody (SAA0667)
- 规格:
50-100ug
抗Bet v 1/Allergen Bet v I-A单克隆抗体(克隆号SAA0667)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

抗Bet v 1/Allergen Bet v I-A单克隆抗体(克隆号SAA0667)更多优势产品欢迎新老客户选购。
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MAB1602 小鼠抗ASFV NS4A单克隆抗体(克隆号1A018)
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文献和实验【进展|热点】Nature:TGF-β并非Th17细胞分化的必要因素
文章来源: Kamran Ghoreschi, Arian Laurence,Xiang-Ping Yang, et al. Generation of pathogenic TH17 cells in the absence of TGF-β signaling. Nature.2010,467: 967–971 Nature今天刚刚刊登的文章。 链接: http://www.nature.com/nature/journal/v467/n7318/abs/nature
抗海带多糖单克隆抗体的制备 一 实验目的 利用单克隆抗体的制备原理和技术,将纯化的重组钙网蛋白片段(CRT39-272)和海带多糖(laminarin)偶联作为抗原免疫小鼠,增强海带多糖的免疫原性,制备抗海带多糖(LAM)的单克隆抗体。 二 实验原理 骨髓瘤细胞在体外培养时能大量无限增殖,但不能分泌特异性抗体,而抗原免疫的B淋巴细胞能产生特异性抗体,但在体外不能无限增殖。将免疫脾细胞与骨髓瘤细胞融合后形成的杂交瘤细胞,继承了两个亲代细胞的特性,既具有骨髓瘤细胞能无限制增殖
可使用。静脉注射(i.v.)则只适合于可溶性抗原及分散的单细胞悬液,且不能使用佐剂,其诱发的免疫应答主要发生在脾脏。此外,在单克隆抗体制备时,亦可用脾脏直接注射或体外免疫方法,尤其对微量抗原比较实用。体外免疫方法也常用于人源单克隆抗体的制备。体外免疫时将脾细胞或外周血淋巴细胞(包括B细胞,T细胞及抗原递呈细胞)与抗原一起作体外培养,然后再与骨髓瘤细胞融合。初次免疫后要经过2—3次以上的免疫加强以保证能形成较高水平的IgC抗体。两次免疫注射之间的时间间隔,一般3—4周比较适合大部分动物,小动物可间隔
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