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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
at-20℃
- 库存:
99
- 抗体英文名:
Anti-Human CD289/TLR9 Monoclonal antibody (5G5)
- 规格:
50-100ug
小鼠抗人CD289/TLR9单克隆抗体(克隆号5G5)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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- M E M 培养基(Invitrogen,Carlsbad)。将1.25×105量级的hMSCs接种于一个T75长颈瓶,在细胞汇聚之前让细胞生长3至4天。在培养期末,培养于不同条件的hMSCs同时用胰蛋白酶/EDTA溶液收集。对细胞计数,并且用下面的公式计算扩增倍数。重新培养时,将1.25×105量级的hMSCs接种于一个新的T75长颈瓶,并用适当的培养基培养。每次传代后对细胞进行计数。 抗体。藻红蛋白(PE)连接的小鼠抗人CD90和CD105的单克隆抗体(mAbs),PE非连接的山
。 实验类型:大多数只标明用于WB、IP、IHC、IF、ChIP的抗体都不适用于流式实验(FC,flow cytometry)。选择标明用于FC实验的抗体,并有引用文献和实验数据图。 克隆号:有一些标记物有多个克隆号,选择文献报道中使用多,荧光标记种类多的克隆号。 厂家和批号:不同厂家或同一厂家生产的不同批号的相同荧光的相同标记物的抗体,荧光染料的强度可能有少许差别,不建议一次实验混合使用。 2、流式抗体荧光标记的方式包括直接标记和间接标记两种。 在流式实验过程中,直标抗体一抗上连接有荧光染料,间标
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