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- 2025年07月10日
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- 保存条件：
at-20℃
- 库存：
99
- 抗体英文名：
Anti-BLAGII/Allergen Bla g II Monoclonal antibody (SAA0612)
- 规格：
50-100ug
抗BLAGII/Allergen Bla g II单克隆抗体(克隆号SAA0612)上海圻明生物优势供应欢迎咨询。配对抗体小课堂：
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.
抗BLAGII/Allergen Bla g II单克隆抗体(克隆号SAA0612)更多优势产品欢迎新老客户选购。
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文献和实验) 。 ( 3 ) 384 孔微量滴定板（Gentix，Christchurch，UK ) 。 ( 4 ) 96 孔深孔板：每孔容积 2 ml 的 96 孔微滴定板（Qiagen，Hilden，Germany) 。 ( 5 ) 96 针复制器（Nunc，Wiesbaden，Germany) 。 ( 6 ) 4 X SB 培养基：48 g/L 胰化蛋白胨，96 g/L 酵母提取物，0.8% ( V/V）甘油，120°C 高压灭菌 20 min。 ( 7 ) 20 X PP 缓冲液（磷酸钾盐缓冲
1. 前言 与其他真核细胞一样，植物细胞中，糖基化通常发生在分泌蛋白质上，虽然在细胞质蛋白和核蛋白上也发现一些糖基化反应。根据寡糖部分和蛋白质骨架之间的连接方式，可将糖基化分为两种类型：N -糖基化和 O-糖基化。植物中 N-糖基化研究最多。 1.1 N-糖基化 与其他真核细胞一样，在植物细胞中，N-糖基化只发生在进入分泌途径的蛋白质上。它从内质网中的寡聚糖前体 Glc3Man9GlcNAC2 共翻译转移到构成初生蛋白质 ( Asn-X-Ser/Thr，X 可以是除脯氨
of saturation mutagenesis to identify and derive consensus sequence(s) for cis-acting-element(s). Identification of the minimal promoter. i & ii, the promoter is spliced to a reporter gene on a plasmid. iii nested deletions with exonuclease iii coupled
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