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- 保存条件：
at-20℃
- 库存：
99
- 抗体英文名：
Anti-Phospho-γ-H2AX (pS139) Monoclonal antibody (G2)
- 规格：
50-100ug
小鼠抗Phospho-γ-H2AX(克隆号pS139)单克隆抗体(克隆号G2)上海圻明生物优势供应欢迎咨询。配对抗体小课堂：
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.
小鼠抗Phospho-γ-H2AX(克隆号pS139)单克隆抗体(克隆号G2)更多优势产品欢迎新老客户选购。
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文献和实验-HCl缓冲液透析，4℃过夜，换液三次。（4）取出标记抗体，用含1%BSA的Tris-HCl缓冲液稀释至4ml，即为AP标记物原液。（5）每毫升中加入0.4ml甘油，小量分装，保存备用。3、PAP、APAAP的制备PAP（过氧化物酶-抗过氧化物酶桥联酶标技术）、APAAP（碱性磷酸酶-抗碱性磷酸酶桥联酶标技术）的制备对于日益广泛使用单抗的免疫细胞化学有重要意义。现在已有商品化的小鼠、大鼠、豚鼠、山羊、绵羊和兔PAP、APAAP试剂供应，只要配以相应的桥抗体，即可非常便利地应用单抗。因为PAP法和APAAP
from the block they synchronously progress through S, G2 , and M. We recently reported that exposure of cells to these inhibitors at concentrations commonly used to synchronize cell populations led to phosphorylation of histone H2AX on Ser 139 (induction of γH2AX
对照 所有的REAfinity抗体都是人IgG1亚型；因此，对于超过1万种REA克隆号的所有抗体来说，只需选用一种类型的同型对照抗体即可。根据抗原表达位置不同，该通用型同型对照抗体分为膜表面型和胞内型两类，无需花费时间寻找合适的同型对照抗体。 图2：使用REAfinity抗体时，只需一种通用型同型对照，降低了实验设计的复杂程度 无需Fc受体阻断 REAfinity抗体Fc段经基因突变改造，从而消除了抗体与Fcγ受体的非特异性结合。因此，在流式分析中，无需额外增加阻断步骤。 优选序列，染色指数高 挑选最佳克隆号的抗体
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