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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
at-20℃
- 库存:
99
- 抗体英文名:
Anti-Human AFP/Alpha-fetoprotein Monoclonal antibody (AF5)
- 规格:
50-100ug
小鼠抗人AFP/Alpha-fetoprotein单克隆抗体(克隆号AF5)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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文献和实验【摘要】 目的: 建立检测甲胎蛋白化学发光免疫定量分析方法. 方法: 采用双抗体异位点一步夹心法,以甲胎蛋白单克隆抗体作为固相包被,采用改良过碘酸钠法进行甲胎蛋白多克隆抗体与碱性磷酸酶偶联制备酶标抗体;以金刚烷胺衍生物CSPD作为底物,优化CSPD与SapphireII发光体系;用国家标准品校定定量标准品,建立了人血清AFP的化学发光免疫定量分析法. 用该法检测800份正常人血清,确定正常值参考范围,将临床血清标本100份与bayer Acs: 180�全自动发光体系统进行
CD11a/CD11b/CD11c 分子 CDlla 常用单克隆抗体或代号:MHM24, 2F12; CRIS―3(LFA―lα链,整合素α1) 主要表达细胞:Leu[A5] 分子质量(kDa)和结构:spl80(整合素α) 功 能:与1CAM-l(CD54)、ICAM-2(CDl02)、ICAM-3(C1350)结合,介导细胞黏附;与JAMl结合,参与白细胞穿过内皮细胞
。 实验类型:大多数只标明用于WB、IP、IHC、IF、ChIP的抗体都不适用于流式实验(FC,flow cytometry)。选择标明用于FC实验的抗体,并有引用文献和实验数据图。 克隆号:有一些标记物有多个克隆号,选择文献报道中使用多,荧光标记种类多的克隆号。 厂家和批号:不同厂家或同一厂家生产的不同批号的相同荧光的相同标记物的抗体,荧光染料的强度可能有少许差别,不建议一次实验混合使用。 2、流式抗体荧光标记的方式包括直接标记和间接标记两种。 在流式实验过程中,直标抗体一抗上连接有荧光染料,间标
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