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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
at-20℃
- 库存:
99
- 抗体英文名:
Anti-GRIA2/GluR-2 Monoclonal antibody (15F1)
- 规格:
50-100ug
小鼠抗Rat GRIA2/GluR-2单克隆抗体(克隆号15F1)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

小鼠抗Rat GRIA2/GluR-2单克隆抗体(克隆号15F1)更多优势产品欢迎新老客户选购。
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文献和实验分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
(2)DNP-KLH预致敏B细胞为DNP-KLH免疫小鼠的脾细胞经抗Thy-1加补体处理去除T细胞而获得。 (3)BGG免疫小鼠脾细胞除去B细胞后为BGG 载体 预致敏T细胞。 (4)将DNP-KLH预致敏B细胞和BGG预致敏T细胞输入经照射后(a*b)F1受体,再用DNP-BGG抗原刺激,检测机体对DNP抗体应答的再次反应。 (二)MHC对Tc杀伤病毒感染靶细胞的约束
(2)DNP-KLH预致敏B细胞为DNP-KLH免疫小鼠的脾细胞经抗Thy-1加补体处理去除T细胞而获得。 (3)BGG免疫小鼠脾细胞除去B细胞后为BGG 载体 预致敏T细胞。 (4)将DNP-KLH预致敏B细胞和BGG预致敏T细胞输入经照射后(a*b)F1受体,再用DNP-BGG抗原刺激,检测机体对DNP抗体应答的再次反应。 (二)MHC对Tc杀伤病毒感染靶细胞的约束
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