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- 文献和实验
- 技术资料
- 保存条件：
at-20℃
- 库存：
99
- 抗体英文名：
Anti-IL2 Monoclonal antibody (S4B6)
- 规格：
50-100ug
兔抗小鼠IL2单克隆抗体(克隆号S4B6)上海圻明生物优势供应欢迎咨询。配对抗体小课堂：
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.
兔抗小鼠IL2单克隆抗体(克隆号S4B6)更多优势产品欢迎新老客户选购。
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文献和实验基础。 传统的NK细胞扩增技术是在NK细胞培养体系中添加IL2、IL15等细胞因子以促进NK细胞增殖，但由于扩增效率不高且纯度不够未能得到广泛应用。近年来，有人采用基因工程的方法在K562细胞上同时表达IL-15、CD137L 等分子, 并以此细胞来刺激NK细胞的增殖。IL-15和CD137L联合刺激是PBMC 中NK细胞特异性扩增的基础：作为膜蛋白，通过细胞间的接触刺激，用以诱导NK细胞扩增。IL15能够促进NK细胞的分化成熟，而IL21能够联合IL15分子促进NK细胞的分化成熟，所以本研究在稳定
Protocol for intracytoplasmic staining of cytokines for FACS analysis
analysis: anti-IL2 (S4B6-FITC; Pharmingen 18004A or home made conjugate) for FL1 anti-TNF-alpha (MP6-XT22-PE; Pharmingen 18135A) for FL2 anti-IFN-gamma (XMG1.2-FITC; Pharmingen 18114A or home made conjugate) for FL1 anti-IL4 (11B11-PE
细胞数量的药物，如环孢霉素A和FK506。这两种药物主要通过抑制钙调磷酸酶的功能而抑制IL2、Ⅱ／-3、IL4、IL8和IFN-γ等细胞因子基因的转录，抑制T细胞的活化。雷帕霉素是鸟苷酸合成酶和双氢乳清酸酯脱氢酶的抑制剂，可以阻断活化T细胞的克隆扩增和终末分化。环孢霉素A、FI 另外可通过制备特异性自身抗原HCgp-39中提取的肽段与可溶性的MHC蛋白的复合物，使特异性T细胞失活；以T细胞受体的蛋白成分为抗原刺激人体产生免疫反应，特异性地抑制自身反应性T细胞。 2．恢复Thl
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