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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
at-20℃
- 库存:
99
- 抗体英文名:
Anti-HRSV-A F/Fusion glycoprotein F0 Monoclonal antibody (Fab13)
- 规格:
50-100ug
抗HRSV-A F/Fusion glycoprotein F0单克隆抗体(克隆号Fab13)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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【摘要】目的 构建抗CD20嵌合抗体Fab'和F(ab)2片段表达载体,并在大肠杆菌中进行高效可溶性分泌表达,比较二者的活性差异。方法 从相应的杂交瘤中提取mRNA建立抗CD20 ScFv 噬菌体显示文库,利用PCR方法从阳性克隆扩增抗CD20抗体轻链可变区基因(VL)、重链可变区基因(VH),重组到表达载体pYZF1,构建抗CD20 Fab’和抗CD20 F(ab)2达载体pYZF1cd20和pYZcpp3,并在16c9菌中高效表达。MTT法测定Fab’和F(ab)2对Raji和Daudi
密切相关。七十年代,研究人员用凝胶梯度电泳等 生物 化学方法发现了几十种血小板表面膜蛋白,其中一组被称为糖蛋白I(glycoprotein I, GPⅠ)复合物的膜蛋白与血小板“粘附”功能有关。这组复合物又可分为GPⅠb-Ⅸ复合物和GPⅠa-Ⅱa 复合物两类。1979年,笔者赴法进修血液学,有机会通过杂交瘤技术,鉴定出国际上第一个抗血小板膜糖蛋白I的单克隆抗体(AN51)。这株单克隆抗体(以下简称“单抗” ),与正常血小板特异性结合后,血小板就不能通过vW因子“粘附”到破损
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