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- 保存条件：
at-20℃
- 库存：
99
- 抗体英文名：
Anti-DENV-2 E-DIII Monoclonal antibody (1A1D)
- 规格：
50-100ug
抗DENV-2 E-DIII单克隆抗体(克隆号1A1D)上海圻明生物优势供应欢迎咨询。配对抗体小课堂：
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.
抗DENV-2 E-DIII单克隆抗体(克隆号1A1D)更多优势产品欢迎新老客户选购。
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MAB1601 小鼠抗ASFV NS4A单克隆抗体(克隆号1A017)
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文献和实验细胞毒性T细胞对靶细胞杀伤的主要机制是通过释放的穿孔素-颗粒酶系统介导的溶靶细胞作用或通过fas/fasL系统介导的细胞凋亡作用。因此，对CTL颗粒酶的胞泌作用分析可反映细胞毒性T细胞功能。该方法操作简便且无需靶细胞作为指示细胞。 一、基本原理 应用直接针对TCR的单克隆抗体，模拟特异性靶细胞的作用，诱导CTL细胞内颗粒丝氨酸酯酶等的释放。抗TCR可与CTL细胞表面TCR的α和β链及CD3复合物特异性结合。该法基本过程是，先用单克隆抗体包被微孔板，然后加入CTL或T
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，用含有10%热灭活胎牛血清的RPMI1640培养基于5% CO2，37C条件下培养。抗CD20单克隆抗体HI47由我所免疫室提供。辣根过氧化酶标记羊抗人IgG，FITC标记的羊抗鼠IgG购于北京中山生物工程公司。2、抗CD20单克隆抗体轻、重链基因的克隆利用RT-PCR法，采用抗体通用的兼并性引物，从HI47杂交瘤细胞特异性地分别扩增抗体轻链、重链可变区基因（VL和VH）后，利用编码短肽（G4S）3的连接链DNA片段，经overlap PCR，将VH和VL基因连接构成ScFv基因（约750bp
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