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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg
| 规格: | 20 µg | 产品价格: | ¥3870.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥8940.0 |
蛋白名称:Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Human CTLA4 (NP_005205.2)(Met1-Phe162) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:1.Immobilized Recombinant Human CD86 / B7-2 Protein (Fc Tag) (Cat:10699-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated (Cat:11159-H41H-B), the EC50 is 3.6-11.5 ng/mL. 2.Immobilized Anti-CTLA4(BMS) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 11159-H41H-B), the EC50 is 0.4-1.2 ng/mL (Routinely tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Lys 36
蛋白分子量:The recombinant Human CTLA4 consists of 386 amino acids and predicts a molecular mass of 42.89 kDa. It migrates as an approximately 53.61 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_005205.2
蛋白氨基酸序列:Met1-Phe162
蛋白标签:C-human IgG1-Fc & AVI
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