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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against synthesized peptide derived from human TF3C3. AA range:101-150
- 形态:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 保存条件:
-20°C/1 year
- 克隆性:
Polyclonal
- 适应物种:
Human,Mouse
- 保质期:
1年
- 宿主:
Rabbit
- 应用范围:
WB,IHC-p,ELISA
- 浓度:
1 mg/ml
- 靶点:
GTF3C3
- 抗体英文名:
TFIIIC102 rabbit pAb
- 抗体名:
General transcription factor 3C polypeptide 3
- 规格:
50ul
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
ELISA Procedure for Measuring Serum Antibody Titer
Dilution ScheduleDilution ofAntiserum Volume of 1% BSA/PBS-T (µL) Volume of PreviousDilution (µL) 1:100 1000 1000 of 1:50 1:200 1000 1000 of 1:100 1:400 1000 1000 of 1:200 1:800 1000 1000 of 1:400 ... ... ... 1:102,400 1000 1000 of 1:51,200 11. Vortex
Diego, CA); GSK-3α (Upstate, LakePlacid, NY); and b-actin (Sigma, St. Louis, MO). 7. Secondary antibodies: anti-mouse and anti-rabbit IgG conjugatedto horseradish peroxidase (Santa Cruz Biotechnology,Santa Cruz, CA). 8. Enhanced chemiluminescent
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