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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from human protein . at AA range: 380-460
- 形态:
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- 保存条件:
-20°C/1 year
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
1年
- 宿主:
Rabbit
- 应用范围:
WB,ELISA
- 浓度:
1 mg/ml
- 靶点:
CYB5R4 NCB5OR
- 抗体英文名:
NB5R4 rabbit pAb
- 抗体名:
Cytochrome b5 reductase 4 (EC 1.6.2.2) (Flavohemoprotein b5/b5R) (b5+b5R) (N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein) (cb5/cb5R)
- 规格:
50ul
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Purification of Antiserum or Ascites by Protein A/G Chromatography
(4) Neutralization Buffer (NB). See recipe below(5) Elution Buffers (pH 2.7 and pH 1.9). See recipes below(6) Centrifuge tubes and microcentrifuge tubes(7) Preparative centrifuge and microcentrifuge(8) pH strips(9) Microtiter plate reader with 600 nm filter(10) Glass
a miniprep as in step 5. (Make -70 stock of bacterial pool for future use). Transform NotI digest of entire pool into yeast, selecting for URA3 . NB For HAT epitope-tagging, you may want to pre-transform your yeast strain with pB227
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