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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic Peptide of Histone H3 (Tri Methyl Lys14)
- 形态:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 保存条件:
-20°C/1 year
- 克隆性:
Polyclonal
- 适应物种:
Human,Rat
- 保质期:
1年
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
1 mg/ml
- 靶点:
HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B
- 抗体英文名:
Histone H3 (Tri Methyl Lys14) rabbit pAb
- 抗体名:
Histone H3.1/Histone H3.2/Histone H3.3
- 规格:
50ul
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文献和实验组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白;在细胞正常 pH 值时,组蛋白带有正电荷,这样它们就可以和 DNA 结合,这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实,组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg , Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白: H1 , H2A , H2B , H3
Installation of Site‐Specific Methylation into Histones Using Methyl Lysine Analogs
properties to their natural counterparts. The ease of synthesis of methyl lysine analog (MLA) histones, especially on a large scale, makes them particularly useful reagents for studying the effects of histone lysine methylation on chromatin structure
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
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