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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic Acetyl peptide from human protein at AA range: 24/25
- 形态:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 保存条件:
-20°C/1 year
- 克隆性:
Polyclonal
- 适应物种:
Human,Rat,Mouse
- 保质期:
1年
- 宿主:
Rabbit
- 应用范围:
WB,ELISA
- 浓度:
1 mg/ml
- 靶点:
Histone H2B
- 抗体英文名:
Histone H2B (Acetyl-Lys24/25) rabbit pAb
- 抗体名:
Histone H2B
- 规格:
50ul
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文献和实验preparation buffer II, prechilled on ice Nuclei preparation buffer III, prechilled on ice Phenol:chloroform:isoamyl alcohol 25:24:1 (v:v:v) For extraction of genomic DNA, the phenol should be saturated beforehand with 100 mM Tris-Cl
duplex PCR amplification, which is the coamplification of a fragment from the region of interest and a control fragment (e.g., the actin gene, or the tubulin gene). This approach allows for estimating relative levels of specific histone modifications
Site-directed hydroxy radical mapping of nucleosome positions in vitro
and Davey, 2003). Procedure Preparation of Derivatised Octamer Refold and purify histone octamer containing histones H2A wild type, H2B wild type, H3 Cys110Ala and H4 Ser47Cys following standard methods (Dyer et al., 2004) (see note 1);
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