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- 2025年12月05日
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文献和实验Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
an alternate approach, the Nucleic Acid Programmable Protein Array (NAPPA) is constructed by spotting protein?encoding plasmid DNA at high density, in addressable fashion, on an array surface. Proteins are subsequently generated in situ
Agarose Gels for Single Stranded DNA
1. Prepare 50X TAE as: 242 g Tris Base 57.1 mL Glacial Acetic Acid 100 mL 500 mM EDTA, pH 8.0 600 mL ddH2O Mix. Bring volume to 1 L. Autoclave. 2. Mix the following: 0.40 g Agarose 4.00 mL 50X TBE (4X TAE final) 46.00 mL
, 2.2M dNTP’s 100mM EDTA 0.5M EtBr 10mg/ml Gelatin 2% Glucose 1; 1.5; 2M Guanidine HCl 1-8M HEPES 1M Imidazol 2M Paraformaldehyde 37% PEG 40% PMSF 100mM Retinoic acid 10mM Sucrose 1; 2; 2.5M Tris Cl 1M
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