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Please store the product under the recommended conditions in the Certificate of Analysis.
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货期:1-2天
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MedChemExpress LLC
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1 mg/5 mg/10 mg
| 规格: | 1 mg | 产品价格: | ¥1550.0 |
|---|---|---|---|
| 规格: | 5 mg | 产品价格: | ¥4380.0 |
| 规格: | 10 mg | 产品价格: | ¥7000.0 |
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
D Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A D8 IgG2b, 09821D Eryth: Ter-119 IgG2b, 09081A Sca-1 PE-IgG2a (E31-161.7) 01835B PE-IgG2a Isotype control, 11025
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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