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文献和实验Peptide Library Construction from RNA-PCR-Derived RNAs
The generation of peptide from messenger RNA (mRNA) provides a convenient source for current proteomic analysis. Intron-free mRNA possessing adenine-uracil-guanine (AUG) start codons can be translated into labeled or unlabeled peptides
Assaying Peptide Translocation by the Peptide Transporter TAP
MHC class I molecules display peptides at the cell surface that are mostly derived from cytosolic or nuclear proteins. Since peptide loading of MHC class I molecules occurs in the ER lumen, cytosolic peptides have to pass the ER membrane
in this area has concentrated on studying the structures of antigen in complex with antibody Fab fragments. In practice, this requires the synthesis of a short peptide or oligosaccharide that binds to the antibody in question and then determination
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