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100 assays
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
that any sample is above 50 ng/mL range, dilutions should be performed using the Assay Buffer provided. 2) Tissue extracts or cell culture samples may require dilution. Dilutions should be performed using the Assay Buffer provided. 2. Pre-warm
MINICHROMOSOME MICROTUBULE BINDING ASSAY
as a positive control for hybridization. The final concentration of supercoiled plasmid should be such that ~0.1ng is loaded. Run gel at 20 or 30V overnight. Photograph gel. Note whether the intensities of the øX174 bands are even in all lanes and whether 2u
. BLOTTO) Random-primed DNA labeling kit Wash solutions (Wash1 2xSSC, 0.1%SDS; Wash2 0.1XSSC, 0.1%SDS) 2.2. RDA and cloning PCR products. 3 X EE buffer : 30 mM EPPS (SIGMA) pH 8.0, 3 mM EDTA pH 8.0. 5 M NaCl cDNA
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