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文献和实验Hydrogen Peroxide Assay for Amine Oxidase Activity
substrates, but are relatively insensitive and technically difficult. Coupled ammonia measurements are also relatively insensitive (8 ) and limited to amine oxidases acting at the primary amino groups. The method described in this chapter is comparable
Primary Neuron Viability Assay
AM (Molecular Probes,OR)1 mM in DMSO. Dilute toa final conentration of 1 micromolar in cell culture growth medium. Supplies Fluorescence plate reader; standard cell culture facilities, supplis and reagents. Tips Perform a timecourse and concetration
Assay of Arachidonic Acid Release Coupled to 1- and 2-Adrenergic Receptors
. 1 . Phospholipase A 2 (PLA 2 ) provides a direct pathway for AA release and is the most common source of cellular AA liberation. PLA 2 is the primary source of free AA in most cell systems, including neurons. Physiological regulation of PLA 2
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