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- MRPA-100
- 2025年07月14日
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文献和实验In Vitro Analysis of the Yeast Mitochondrial RNA Polymerase
in vitro with this purified enzyme in transcription reactions. These assays are critical for elucidating the nature of a mitochondrial promoter and for understanding how the mitochondrial RNA polymerase recognizes these DNA sequences and selectively
Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease
of mitochondrial diseases with more than 100 known disease mutations. Thus, we need to understand how and why pol γ defects lead to disease. By using an extensive array of methods, we are developing a clearer understanding of how defects in pol γ contribute
The Polymerase Chain Reaction (PCR)
by the integrity of the starting target DNA — > 50 kb. Can I PCR Amplify RNA? • Not directly — the DNA polymerase requires a DNA template and will not copy RNA. • mRNA can first be copied into cDNA using reverse transcriptase. • cDNA is a template for PCR —
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