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文献和实验Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
, A.R., Barnum, J.E., Harmon, L.S., DeLuca, J.G., and Nichols, W.W. 1994. Rapid DNA degradation in primary rat hepatocytes treated with diverse cytotoxic chemicals: Analysis by pulsed‐field gel electrophoresis and implications for alkaline elution assays
Methylation and Uracil Interference Assays for Analysis of Protein‐DNA Interactions
between E. coli RNA polymerase and an early promoter of phage T7. Proc. Natl. Acad. Sci. U.S.A. 77:122‐126. Key References
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