- ￥5901.83
- Profoldin
- DNAB-100EC
- 2025年07月11日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 规格：
100 assays
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Transformation of E. coli by Electroporation
100% ethanol 70% ethanol 0.5X TE SOC medium transformation plates I. Preparation of E. coli cells
Maxiprep of plasmid DNA from E. coli
Grow a single colony of E. coli overnight in 50mL LB broth + selective markers at 37°C. The next morning, put 850μL of the culture in each of two Eppendorf tubes, add 150μL sterile 50% glycerol, and store at -80°C. Pour as much culture as will fit
Transformation DNA fragments (or plasmid DNA) into competent E. coli
Transformation DNA fragments (or plasmid DNA) into competent E. coli * Caution: Use aerosol protecting tips if selection of transformed cells is not based on X-gal strategy. 1.Remove competent cells (E.coli DH5aTM from GIBCO BRL) from -70 ℃
技术资料暂无技术资料 索取技术资料
