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文献和实验Introduction to the EMSA (Gel Shift) Technique
to resolve very large complexes, as is the case with E. coli RNA polymerase (~460 kDa). Gels are pre-run at a constant voltage until the current no longer varies with time. The primary reasons for pre-running gels is to remove all traces of ammonium
Introduction to the EMSA (Gel Shift) Technique
to resolve very large complexes, as is the case with E. coli RNA polymerase (~460 kDa). Gels are pre-run at a constant voltage until the current no longer varies with time. The primary reasons for pre-running gels is to remove all traces of ammonium
A Homogeneous Assay to Assess GABA Transporter Activity
., Sigma) E. coli DH5α (Invitrogen) Collagen‐coated Cytostar‐T scintillating microplates (see recipe
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