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文献和实验E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow-through liquid and wash the column by adding 700 u DNA Wash Buffer diluted with ethanol
Strategies to Optimize Protein Expression in E. coli
that Influence Expression and Solubility III. Properties of the Vector that Influence Expression and Solubility IV. E. coli Host Strains that aid Expression of Heterologous Proteins V
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
restriction enzyme (e.g., EcoRI). Third, intramolecular ligation after cross-linking and digestion will lead to a fraction of the DNA occurring as circular DNA that cannot be cloned; this might further reduce the number of clones in such an assay. Finally
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