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- Profoldin
- DNAB500KP
- 2025年07月10日
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999
- 规格：
500 assays
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文献和实验volume=approximately 500 microliters.) 12, Add 20 microliters 5M NaCl to the combined eluates (500 microliters) and reverse histone-DNA crosslinks by heating at 65�C for 4 hours. Note: Include the input DNA from this step.� 13, Add 10
2.Quantitate Protein Concentration Quantitate proteins in 15 ml aliquots of lysates (in duplicate)by using a Bradford assay (I have been using the Biorad kit),using BSA as standard.Don't forget to add 15 ml LB to each BSA sample and to the blank tube
Chromatin IP (CHIP assay) This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. [1997, Gen & Dev 11, p83-93] and was obtained from Flick K. (The Scripps Research Institute).
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