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文献和实验Rapid Method for Preparation of Genomic DNA from P. aeruginosa
TNE buffer. Add 135 µl of TNE buffer containing 2% Triton X-100. Add 30 µl freshly prepared lysozyme (5 mg/ml). Mix well by tapping the tube. Incubate in a 37℃ water bath for 30 minutes. Add 15 µl proteinase K solution (20 mg/ml). Mix
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
Pseudomonas aeruginosa Microarray Protocol
, however when treated as RNase-free and only used for RNA related procedures, they have worked well. · 15 mL conical tubes, Corning #430052 · 100% Isopropanol, Mallinckrodt AR
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