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文献和实验Activating the NLRP3 Inflammasome Using the Amyloidogenic Peptide IAPP
an inflammasome complex that ultimately cleaves pro-IL-1β and pro-IL-18 into their mature, secreted forms. Here we describe a protocol by which one type of amyloidogenic peptide, islet amyloid polypeptide (IAPP, otherwise known as amylin) can be prepared and used
Metabolic Assessment in Alamethicin-Activated Liver Microsomes: Co-activating CYPs and UGTs
The methods and materials described in this chapter are for a medium throughput screening assay for the study of parallel CYP- and UGT-mediated metabolic pathways in microsomes. Alamethicin, a pore-forming peptide, was used to activate UGTs
Methods for analysis of matrix metalloproteinase regulation of neutrophil-endothelial cell adhesion
neutrophil-endothelial cell attachment. ET-1[1–32] produced by MMP-2 up-regulated CD11b/CD18 expression on human neutrophils, thereby promoted their adhesion to cultured endothelial cells. ET-1[1–32] evoked release of gelatinase B (MMP-9), which in turn
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