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- Cedarlane
- CLY100-187AF-500UG
- 2025年07月12日
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文献和实验Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
at 200 x g for 4 minutes. Remove the supernatant and gently flick the pellet. Resuspend the pellet in an appropriate amount of Human Feeder Cell Conditioned Medium supplemented with 4 ng/mL of recombinant human FGF basic. Add
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
β-mercaptoethanol, 1000X Gibco® Mouse Embryonic Fibroblasts (Irradiated) Basic Fibroblast Growth Factor (bFGF) GlutaMAX™-I (100X) Collagenase Type IV for enzymatic passaging or StemPro® EZPassage™ Tool for mechanical
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
(approximately 1.0 x 106 cells/60 mm plate) on the Cultrex BME coated plate in Conditioned Media containing 4 ng/mL of recombinant human FGF basic. Change the media daily. Monitor the cells for the desired confluency. Figure
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