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文献和实验Purification of Growth Factor mRNA in Renal Tissues:bFGF-2, FGF-2, TGFα, and EGFR
benign and cancerous human and animal tissues, including kidney, prostate, and bladder (3 –6 ). In some cases it has also been demonstrated to have potential as a tumor marker (7 –11 ). One group reported greater recovery of both FGF-2 protein and FGF-2
Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions
with animal derived antibodies, and are maintained on mouse embryonic feeder (MEF) cells, in the presence of either fetal calf serum (FCS) or on Matrigel or with conditioned media from MEFs. Successful derivation of hESCs in xeno-free conditions is crucial
Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions
and future clinical application require an animal-free and well-defined culture system for hESCs, these conventional conditions would prevent the use of hESCs in human therapy. This chapter describes optional culture conditions based on either animal-free
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