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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
The production of monoclonal antibody by hybridoma
changing of the media at day 8 (+/- 150 ml). Should see macroscopic colonies at this time. At this time can decrease the HCF to 0.5% (gradually- 2%, then 1%, then 0.5%) in the cloning plates. 17. Isotype via supernatants
of a mouse-human chimeric monoclonal antibody to Cd20 with potent Fc-dependent biologic activity. J.Immunol.,1987,139:3521[4]Press ow,Farr AG,Borroz KI,et al. Endocytosis and degradation of monoclonal antibodies targeting human B-celll malignancies. Cancer
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