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文献和实验Basic Method for Direct Immunofluorescence Labeling
Antibodies: Test antibody: Mouse monoclonal conjugated to FITC, PE, PerCP or PE-CY5 Tandem (tradenames: Cy-Chrome or Tri-Color). If you're planning to label cells with 2 or more antibodies simultaneously, you need a negative control for each fluorochrome
. You can see how complicated this can become... The best solution for simultaneous labeling is get all direct conjugates, if at all possible. Then see our our protocol for direct labeling. Materials Primary Antibodies: Test antibody: Usually mouse monoclonal IgG or IgM
Basic Method for Direct Immunofluorescence Labeling
of labeling with different antibodies. If any of the above apply, see our protocol for indirect labeling . Materials Fluorochrome-Conjugated Antibodies: Test antibody: Mouse monoclonal conjugated to FITC
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