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文献和实验A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
The production of monoclonal antibody by hybridoma
of ~5ug of antigen in PBS i.p. or intravenously in tail vein of the mouse (with high titer already determined). 1. Spin down myelomas and wash with 30 ml serum free media (CMNS has glutamine). Use tabletop centrifuge at 850
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
al. 2006b ). MATERIALS Reagents Anti-CD24 monoclonal antibody (mAb) (J11d clone) (for Protocol 3) Use either culture supernatant that contains anti-CD24 mAb or purified anti-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing
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