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文献和实验The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
; CD15); Apo1-FAS antigen (tumor necrosis factor superfamily member 6, TNFRSF6; CD95); integrin- 6 (ITGA6; CD49f) and integrin- 4 (ITGB4; CD104); the adhesion molecules CD54 (intracellular adhesion molecule, ICAM-1) and leukocyte function-associated
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