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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
【翻译】Development trends for monoclonal antibody cancer therapeutics
, first described in 1975 (Ref 6), involved use of mouse-derived hybridomas. However, in humans, murine mAbs were commonly (although not always) ineffective as cancer therapeutics. Studies revealed that murine candidates often had short circulating half-lives
The production of monoclonal antibody by hybridoma
and grow up for ascites/ large flask production and further freeze down. Troubleshooting I strongly reccommend to use Southern Biotech Goat anti-Mouse Ig (H+L
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