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文献和实验Immunohistochemisty-Fluorescence Protocol
Materials Cytokine-specific Primary Antibodies unlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'' or ''BAF'' series) or selected monoclonal antibodies. Secondary Antibodies
Immunohistochemistry: Fluorescence Protocol 2.0
Immuno Research Lab Catalog # 705-066-147) Biotinylated goat anti-mouse IgG1 ( Caltag Lab Catalog # M32115) FITC-labeled goat anti-mouse IgG1 ( Caltag Lab Catalog # M32001) FITC-labeled anti-mouse IgG2A ( Caltag Lab Catalog # M
by a non-relevant immunoglobulin (i.e, normal serum for polyclonal antibodies, control mouse ascites fluid for ascites, and isotype controls for purified mouse monoclonal antibodies). 3) Proteolytic digestion can occur when cells are lysed and contents
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