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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
This note is not intended as a full protocol for making monoclonal antibodies, but is to give a few hints and tips for the use of Y3/Ag1.2.3. Generally speaking the methodology is similar to making murine
The production of monoclonal antibody by hybridoma
of ~5ug of antigen in PBS i.p. or intravenously in tail vein of the mouse (with high titer already determined). 1. Spin down myelomas and wash with 30 ml serum free media (CMNS has glutamine). Use tabletop centrifuge at 850
Immunohistochemisty-Fluorescence Protocol
FITC-labeled anti-mouse IgG2A (Caltag Lab Catalog # M32201) FITC-labeled anti-mouse IgG2B (Caltag Lab Catalog # M32401) Oregon Green coupled anti-mouse IgG (Molecular Probes Catalog # 06380) Oregon Green-avidin D(Molecular Probes
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