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文献和实验Restriction endonuclease digestion A six-cutter (e.g. Eco RI) will cut on average every 4.1 Kb . Complete digestion of human DNA with this type of enzyme will result in approximately 1 x 106 unique fragments. What is the probability
Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
). Day 2 14. If you are not using rabbit polyclonal antibodies (ie. monoclonal antibodies, ect), add 1 ug of an appropriate secondary antibody (rabbit) and incubate for an additional 1 hour. 15. Add
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
rabbit polyclonalsFRP-1 antisera directed against either the full-lengthprotein or peptide segments; alternatively, one can use commerciallyavailable reagents such as a goat polyclonal antiserum(sc-7425; Santa Cruz Biotechnology, Santa Cruz, CA
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