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- Cedarlane
- CLX246AP
- 2025年07月12日
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
proteins / chromatin proteins: DNaseI (f.c) 1 µg/ µl 2.5 mM MgCl2 RIPA Buffer 10 mM Tris/Cl pH 7.5 150 mM NaCl 5 mM EDTA 0.1% SDS 1% Triton X-100 1% Deoxycholate 1 mM PMSF
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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