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文献和实验Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
The production of monoclonal antibody by hybridoma
of ~5ug of antigen in PBS i.p. or intravenously in tail vein of the mouse (with high titer already determined). 1. Spin down myelomas and wash with 30 ml serum free media (CMNS has glutamine). Use tabletop centrifuge at 850
In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
. Chan, W.Y. and Tam, P.P.L. 1988. A morphological and experimental study of the mesencephalic neural crest cells in the mouse embryo using wheat germ agglutinin‐gold conjugate as the cell marker. Development
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