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文献和实验viral immunity pathogenesis group
., Schroder, W., Zirngibl, H. and Nieswandt, B. (2000). Structural and functional characterization of the mouse von Willebrand factor receptor GPIb-IX with novel monoclonal antibodies . Blood 95(3): 886-93. Bizzaro, N. and Fiorin, F. (1999
Polyclonal and Monoclonal Antibodies
1) After collecting, blood should be allowed to clot for 60 min . at 37 C or O.N at 4 C. 2) Separate the clot from the sides of the tube (ringing) using a pasteur pipette. Place clot at 4 C O.N. 3) Spin at 10,000 x g for 10 min. at 4 C to separate
Polyclonal and Monoclonal Antibodies(克隆和克隆抗体)
injection: 2 weeks later; boost with 15 - 50 ug of Antigen in adjuvant. (If time permits, boost again a month later). 3) 7 - 10 days later bleed the mouse and test serum using the assay which will be used for screening. If titre is not high enough, boost
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